LOCUS       40924_21414       12485 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pGETS109 DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12485)
  AUTHORS   Tsuge K, Matsui K, Itaya M.
  TITLE     One step assembly of multiple DNA fragments with a designed order 
            and orientation in Bacillus subtilis plasmid
  JOURNAL   Nucleic Acids Res. 31 (21), E133 (2003)
  PUBMED    14576333
REFERENCE   2  (bases 1 to 12485)
  AUTHORS   Itaya M, Tsuge K, Matsui K.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-AUG-2003) Mitsuhiro Itaya, Mitsubishi Kagaku Institute
            of Life Sciences; 11 Minamiooya, Machida, Tokyo 194-8511, Japan 
            (E-mail:ita@libra.ls.m-kagaku.co.jp, Tel:81-42-724-6254, 
            Fax:81-42-724-6317)
REFERENCE   3  (bases 1 to 12485)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 12485)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2003"; volume: "31"; issue: "21"; pages: "E133"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (13-AUG-2003) Mitsuhiro Itaya, Mitsubishi Kagaku Institute of Life 
            Sciences"; volume: " 11 Minamiooya, Machida, Tokyo 194-8511, Japan 
            (E-mail:ita@libra.ls.m-kagaku.co.jp, Tel:81-42-724-6254, Fax"; 
            pages: "81-42-724-6317"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..12485
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        10..38
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein
                     gene"
     CDS             86..1273
                     /label=TcR
                     /note="tetracycline efflux protein"
     regulatory      2175..2202
                     /gene="tetL"
                     /standard_name="bsr promoter"
                     /label=bsr promoter
                     /regulatory_class="promoter"
     regulatory      2175..2180
                     /gene="tetL"
                     /standard_name="bsr promoter"
                     /label=bsr promoter
                     /regulatory_class="minus_35_signal"
     regulatory      2197..2202
                     /gene="tetL"
                     /standard_name="bsr promoter"
                     /label=bsr promoter
                     /regulatory_class="minus_10_signal"
     regulatory      2481..2485
                     /gene="tetL"
                     /regulatory_class="ribosome_binding_site"
     CDS             2493..3866
                     /label=TcR
                     /note="tetracycline efflux protein"
     terminator      3938..4024
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      4116..4143
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     gene            4411..7265
                     /gene="repA"
                     /label=repA
     regulatory      4411..4439
                     /gene="repA"
                     /standard_name="spac-1"
                     /label=spac-1
                     /regulatory_class="promoter"
     regulatory      4411..4416
                     /gene="repA"
                     /standard_name="spac-1"
                     /label=spac-1
                     /regulatory_class="minus_35_signal"
     regulatory      4434..4439
                     /gene="repA"
                     /standard_name="spac-1"
                     /label=spac-1
                     /regulatory_class="minus_10_signal"
     protein_bind    4446..4466
                     /gene="repA"
                     /standard_name="lacO"
                     /label=lacO binding site
                     /bound_moiety="LacI"
     protein_bind    4448..4464
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     regulatory      6015..6044
                     /gene="repA"
                     /regulatory_class="promoter"
     regulatory      6015..6019
                     /gene="repA"
                     /regulatory_class="minus_35_signal"
     regulatory      6039..6044
                     /gene="repA"
                     /regulatory_class="minus_10_signal"
     regulatory      6062..6066
                     /gene="repA"
                     /regulatory_class="ribosome_binding_site"
     CDS             6075..7265
                     /codon_start=1
                     /gene="repA"
                     /product="RepA protein"
                     /label=repA
                     /protein_id="BAD00174.1"
                     /translation="MNNEPVKRGKKNRWELNLPIMTYVVADDWIDKLGHETFTLWLRFH
                     TWVDREDELRDYDRIPRSFENIYKKTLGISKSKFYRLIKPLWEYGLIDIIEYEESNRNS
                     TKPKNIIVYEYPLHEIERKYKPLEKLRDWDKDYNSVSKELGKTGGRPRKKDSEEEPEKK
                     PEEVTKKKRKYKLKRVIHNGFKNETVEGFKNETVEGFKNETVTVSKIKPNNYSNIFNNL
                     SNISTNVSNNLLIDDDEEIENEPTGRTINRSLLFSQEDIKQAYQFINRFSVIQLRENFS
                     FDKHFEERLVCYLWKAGISTFYTHEISKMIKKIADYEKSKKGRLNPIRDRALYMVNGLV
                     MNRASSQSEHATYKLNQYKKQKEQEKQQQEQQRSRVPFYNWLEEREEQTEGQLPTT"
     RBS             7602..7610
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     regulatory      8885..8913
                     /gene="lacI"
                     /standard_name="penP promoter"
                     /label=penP promoter
                     /regulatory_class="promoter"
     regulatory      8885..8890
                     /gene="lacI"
                     /standard_name="penP promoter"
                     /label=penP promoter
                     /regulatory_class="minus_35_signal"
     regulatory      8908..8913
                     /gene="lacI"
                     /standard_name="penP promoter"
                     /label=penP promoter
                     /regulatory_class="minus_10_signal"
     regulatory      9062..9069
                     /gene="lacI"
                     /standard_name="penP RBS"
                     /label=penP RBS
                     /regulatory_class="ribosome_binding_site"
     CDS             9079..10158
                     /label=lacI
                     /note="lac repressor"
     misc_feature    10332..10472
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(10658..11246)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(11420..12277)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(12278..12382)
                     /label=AmpR promoter
ORIGIN
        1 ttctcatgtt tgacagctta tcatcgataa gctttaatgc ggtagtttat cacagttaaa
       61 ttgctaacgc agtcaggcac cgtgtatgaa atctaacaat gcgctcatcg tcatcctcgg
      121 caccgtcacc ctggatgctg taggcatagg cttggttatg ccggtactgc cgggcctctt
      181 gcgggatatc gtccattccg acagcatcgc cagtcactat ggcgtgctgc tagcgctata
      241 tgcgttgatg caatttctat gcgcacccgt tctcggagca ctgtccgacc gctttggccg
      301 ccgcccagtc ctgctcgctt cgctacttgg agccactatc gactacgcga tcatggcgac
      361 cacacccgtc ctgtggatcc tctacgccgg acgcatcgtg gccggcatca ccggcgccac
      421 aggtgcggtt gctggcgcct atatcgccga catcaccgat ggggaagatc gggctcgcca
      481 cttcgggctc atgagcgctt gtttcggcgt gggtatggtg gcaggccccg tggccggggg
      541 actgttgggc gccatctcct tgcatgcacc attccttgcg gcggcggtgc tcaacggcct
      601 caacctacta ctgggctgct tcctaatgca ggagtcgcat aagggagagc gtcgaccgat
      661 gcccttgaga gccttcaacc cagtcagctc cttccggtgg gcgcggggca tgactatcgt
      721 cgccgcactt atgactgtct tctttatcat gcaactcgta ggacaggtgc cggcagcgct
      781 ctgggtcatt ttcggcgagg accgctttcg ctggagcgcg acgatgatcg gcctgtcgct
      841 tgcggtattc ggaatcttgc acgccctcgc tcaagccttc gtcactggtc ccgccaccaa
      901 acgtttcggc gagaagcagg ccattatcgc cggcatggcg gccgacgcgc tgggctacgt
      961 cttgctggcg ttcgcgacgc gaggctggat ggccttcccc attatgattc ttctcgcttc
     1021 cggcggcatc gggatgcccg cgttgcaggc catgctgtcc aggcaggtag atgacgacca
     1081 tcagggacag cttcaaggat cgctcgcggc tcttaccagc ctaacttcga tcattggacc
     1141 gctgatcgtc acggcgattt atgccgcctc ggcgagcaca tggaacgggt tggcatggat
     1201 tgtaggcgcc gccctatacc ttgtctgcct ccccgcgttg cgtcgcggtg catggagccg
     1261 ggccacctcg acctgaatgg aagccggcgg cacctcgcta acggattcac cactccaaga
     1321 attggagcca atcaattctt gcggagaact gtgaatgcgc aaaccaaccc ttggcagaac
     1381 atatccatcg cgtccgccat ctccagcagc cgcacgcggc gcatctcggg cagcgttggg
     1441 tcctggccac gggtgcgcat gatcgtgctc ctgtcgttga ggacccggct aggctggcgg
     1501 ggttgcctta ctggttagca gaatgaatca ccgatacgcg agcgaacgtg aagcgactgc
     1561 tgctgcaaaa cgtctgcgac ctgagcaaca acatgaatgg tcttcggttt ccgtgtttcg
     1621 taaagtctgg aaacgcggaa gtcagcgccc tgcaccatta tgttccggat ctgcatcgca
     1681 ggatgctgct ggctaccctg tggaacacct acatctgtat taacgaagcg ctggcattga
     1741 ccctgagtga tttttctctg gtcccgccgc atccataccg ccagttgttt accctcacaa
     1801 cgttccagta accgggcatg ttcatcatca gtaacccgta tcgtgagcat cctctctcgt
     1861 ttcatcggta tcattacccc catgaacaga aatccccctt acacggaggc atcagtgacc
     1921 aaacaggaaa aaaccgccct taacatggcc cgctttatca gaagccagac attaacgctt
     1981 ctggagaaac tcaacgagct ggacgcggat gaacaggcag acatctgtga atcgcttcac
     2041 gaccacgctg atgagcttta ccgcagaatt cgcccttccc ggtcgatatg aacagcttat
     2101 ttacataatt cacgttattg gtagttataa atgaaattcc taatatcggt tatgaagtga
     2161 aattgaattt ctacttgatc tttctctcta tttttgtaaa ataaaattaa gaatatttaa
     2221 atattcaatg attcattttt gcagaaatcg gaggaagaag aatatatgaa aacatttaac
     2281 atttctcaac aagatccccc catattgttg tataagtgat gaaatactga atttaaaact
     2341 tagtttatat gtggtaaaat gttttaatca agtttaggag gaattaatta tgaagtgtaa
     2401 tgaataatga atgtaacagg gttcaattaa aagagggaag cgtatcatta accctataaa
     2461 ctacgtctgc cctcattatt ggagggtgaa atgtgaatac atcctattca caatcgaatt
     2521 tacgacacaa ccaaatttta atttggcttt gcattttatc tttttttagc gtattaaatg
     2581 aaatggtttt gaacgtctca ttacctgata ttgcaaatga ttttaataaa ccacctgcga
     2641 gtacaaactg ggtgaacaca gcctttatgt taaccttttc cattggaaca gctgtatatg
     2701 gaaagctatc tgatcaatta ggcatcaaaa ggttactcct atttggaatt ataataaatt
     2761 gtttcgggtc ggtaattggg tttgttggcc attctttctt ttccttactt attatggctc
     2821 gttttattca aggggctggt gcagctgcat ttccagcact cgtaatggtt gtagttgcgc
     2881 gctatattcc aaaggaaaat aggggtaaag catttggtct tattggatcg atagtagcca
     2941 tgggagaagg agtcggtcca gcgattggtg gaatgatagc ccattatatt cattggtcct
     3001 atcttctact cattcctatg ataacaatta tcactgttcc gtttcttatg aaattattaa
     3061 agaaagaagt aaggataaaa ggtcattttg atatcaaagg aattatacta atgtctgtag
     3121 gcattgtatt ttttatgttg tttacaacat catatagcat ttcttttctt atcgttagcg
     3181 tgctgtcatt cctgatattt gtaaaacata tcaggaaagt aacagatcct tttgttgatc
     3241 ccggattagg gaaaaatata ccttttatga ttggagttct ttgtggggga attatatttg
     3301 gaacagtagc agggtttgtc tctatggttc cttatatgat gaaagatgtt caccagctaa
     3361 gtactgccga aatcggaagt gtaattattt tccctggaac aatgagtgtc attattttcg
     3421 gctacattgg tgggatactt gttgatagaa gaggtccttt atacgtgtta aacatcggag
     3481 ttacatttct ttctgttagc tttttaactg cttcctttct tttagaaaca acatcatggt
     3541 tcatgacaat tataatcgta tttgttttag gtgggctttc gttcaccaaa acagttatat
     3601 caacaattgt ttcaagtagc ttgaaacagc aggaagctgg tgctggaatg agtttgctta
     3661 actttaccag ctttttatca gagggaacag gtattgcaat tgtaggtggt ttattatcca
     3721 tacccttact tgatcaaagg ttgttaccta tggaagttga tcagtcaact tatctgtata
     3781 gtaatttgtt attacttttt tcaggaatca ttgtcattag ttggctggtt accttgaatg
     3841 tatataaaca ttctcaaagg gatttctaaa tcgttaaggg atcaactttg ggagagagtt
     3901 caaaattgat ccttttttta taacaggaat tgggcatcaa ataaaacgaa aggctcagtc
     3961 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctcc tgagtaggac
     4021 aaatccgccg ggagcggatt tgaacgttgc gaagcaacgg cccggagggt ggcgggcagg
     4081 acgcccgcca taaactgcca ggcatcaaat taagcagaag gccatcctga cggatggcct
     4141 ttttgcgttt ctacaaactc ttcctgtcgt catatctaca attctacaca gcccagtcca
     4201 gactattcgg cactgaaatt atgggtgaag tggtcaagac ctcactaggc accttaaaaa
     4261 tagcgcaccc tgaagaagat ttatttgagg tagcccttgc ctacctagct tccaagaaag
     4321 atatcctaac agcacaagag cggaaagatg ttttgttcta catccagaac aacctctgct
     4381 aaaattcctg aaaaattttg caaaaagttg ttgactttat ctacaaggtg tggcataatg
     4441 tgtggaattg tgagcggata acaattaagc taattctatg gtaaaagcat tcctgaaccc
     4501 aatatcctta tttttagtgg acaaggcctt gttctcatat ggttgcttga gccagtgcct
     4561 tacaaggctc ttccgctttg gcaggctgtt caaaattatt tattagagca actcaaggaa
     4621 ctaggcggag atccaaaagc aaccgatgca gcacgtgttt ttcggattgc tgggagcgtc
     4681 aatagtaaga atggagcgga agtaagagcg gaatatcggc acgattacag atatgagtta
     4741 aggcagattc aatttgatta ccttccagaa ctcaatgagg ttattaatcc cgctccacaa
     4801 aagaaaaaac gggggcgtaa aaagaaagta gttcagttat tcaataccta caaactccat
     4861 tacgctcgcc tattggatat agtgaaattg gtggaactac gaaattatga ggtaactgga
     4921 tacagggaaa tcatttgctt tttataccgt tattggttgt gctgttacac gaacgaccct
     4981 gtagaggctt taaatcaaac acagacactt aatttacagt tcactgagcc tttaccctta
     5041 aaagaggttg aaagggctac acggagcgca gaaaaggcgt gggaagcacg aaataacgaa
     5101 gaggctaacc ggattgcaat cgaaaaagga tatcccagtg ctggatacaa tattagcaat
     5161 aaaaagttga ttgaatggct agatatcaca ccagaagaac agaagcattt acagacgatt
     5221 atagacgcta atgagaaacg gagaagaaag cgggaaaggg ataggattta tcaagaacaa
     5281 aaacgtcgtg agcggggaga tatgacccga caggagtata ttaagcaaca acaggataaa
     5341 acagatgata aattatttaa attgcaggag ttattagaac agaatcccaa aatctcaaaa
     5401 attaaattag caaaaatact tggtgtagat aaatcgcact tatacagact gctgaaacaa
     5461 ctataactgg aaaaaattag tgtctcatgg ttcgtcgccc ttatattatg ggcgttagcc
     5521 tgcttgctaa ttggataggt gttattgtca tttcaacgtc ggacgttttg gtagatgaac
     5581 gtcggacgtt atttgcttca atagattgtt gatgtattaa gggttgcagt gaatcgacaa
     5641 gcaaaaagtt atgacgttgt gaaaaaattg aatagaaaat tggaaattac gtcggacgtt
     5701 ctaacttaaa aaccctgtta tatcaatgat ttaaaaggaa attaacgtca taaaagacct
     5761 ttctgcaaca aaagtttttc tggaagagtt gaggttattt tataggtatt atggactttt
     5821 gtagactttt tgtgtacttt ttgtggactc cacactcgct ggtatcgtgt tattttttaa
     5881 ttgagatatg aatatggaaa ttaaacgttt taggcgttgg tttggtgatg acaaaaaaat
     5941 aagagtaccc gctcacaacg gatactcttt cgagaaatgt acgaaacgct ataaataaaa
     6001 aataactaga tacatttaca ttgtatcacg tttcgtacat ttctccaata acaaattgat
     6061 tggaggaatg caaagtgaat aatgaaccag taaaacgtgg taagaagaac agatgggaat
     6121 taaacctacc tataatgact tatgtagtag ctgatgattg gattgataaa ctaggacacg
     6181 aaacgtttac tttatggttg aggttccata cttgggtaga tagagaagat gaactccgag
     6241 attatgatcg catacctaga agttttgaga acatatataa aaagacacta ggaatctcaa
     6301 aaagtaagtt ttatagattg ataaaacctt tatgggaata tggattaata gacatcatag
     6361 aatacgaaga atctaaccgt aattctacta aacctaaaaa tataattgtt tatgagtatc
     6421 ctttacacga aatagaaaga aagtataaac cactagaaaa attaagagat tgggataaag
     6481 actataattc cgtttctaaa gaattaggta aaacaggtgg tagaccaagg aaaaaagata
     6541 gtgaagaaga acccgaaaag aaacccgaag aagtaactaa aaagaaacgt aaatataagt
     6601 taaaaagagt tatccacaac ggtttcaaaa atgaaacggt ggagggtttc aaaaatgaaa
     6661 cggtggaggg tttcaaaaat gaaacggtga ccgtttcaaa aataaaaccc aataattatt
     6721 caaatatctt taataactta tcaaatattt ctactaatgt ttcaaataat ttattaattg
     6781 atgatgatga ggaaatcgaa aatgaaccaa ctggtcgtac aataaatagg tcattacttt
     6841 tttcacaaga agatattaaa caggcctatc aatttattaa tagattttca gttatacagt
     6901 tacgtgaaaa ctttagcttt gataaacact ttgaagaacg gttggtatgt tatttatgga
     6961 aagcagggat ttctactttt tacacgcacg aaatcagtaa aatgataaaa aaaatagcag
     7021 actatgaaaa atctaaaaaa ggtagattaa acccaatacg tgaccgagcc ttatatatgg
     7081 taaatggtct tgtaatgaat agagcttctt cccaaagtga acatgctact tataaactaa
     7141 accaatataa aaaacagaag gaacaggaaa aacaacaaca ggagcaacaa agatcaagag
     7201 taccgttcta taattggttg gaggaaagag aagaacaaac cgaaggtcaa ctacccacca
     7261 cttaaagcct aacggctttt gaagtggggc ttgtaaaaag ccctagttga ctaccccaag
     7321 tctttcgagg actacgttgg aaaggtcatg acacctacaa atgctcctct agttcgtagc
     7381 cactgtcgtt gatggttaaa agtcctgatg ggtagggacg gtgctgtcaa catcacaagc
     7441 ccttccaaca tgggggaaga ggaagaacac tccgagaaag gagggggctg tcgtacacgc
     7501 cttgttcgtg tacgtgattt ttaacacttt tatctctaat ataatctctc cttgagttag
     7561 cagagactaa gaataaaaca cattaggatt ttttatctac ataaggaggt tcaatacatg
     7621 cctactattt cgtttaaggg aaagtctttt gtacaaaatc atcacttgac tgtaaaatat
     7681 catgaattga tcccagaacc agaaaaaagt ctgaccgata aagttagtct tcatgacaat
     7741 cttatcattc atggggacaa tttaaaggcg ttgaaagctc tactacctac atatgctggt
     7801 aagatagatt gcatctatat tgacccacca tacaatactg gaactgaaaa atggatttat
     7861 aatgataatg tcaactcccc aatgattagg gaatggcttg gaaaagtggt agacaaagaa
     7921 gacttatccc gacatgataa gtggctttgt atgatgatgc cgaggttaaa actgttgaaa
     7981 gagttgttgt cagaagacgg agtgatattt gtaagcattg actacaatga aatacaccat
     8041 cttacgtgcc ttatgaacga aatattcggt gaggagaatt ttcgtgacgc tattatcatt
     8101 cgtagaggag taaaaaatgt tcaagcgcaa tttgacacaa ttgattctct ttcaaatggt
     8161 tatgagtcta ttttggttta cactaaacag ccatctcgac gatttaataa ggtttatgaa
     8221 gatgtagaag aaaagcctgg tagttggaat aatcattgga gaggtacaga ccgtaaaact
     8281 atgagatatg aattatttgg ggttactccg acagaaggtc aatggagatg ggggaaagaa
     8341 cgtagtctcc aggctattga aaactatcaa atgatgttag atgaacttag acaaaaaggc
     8401 attactaatc caactcaaga ggatattgat aaatggtatc ttgaatatgt agaaaataat
     8461 ggggaagata ttgacttgtt acgacaaaat gataatggca cgattgagca ctttgttcca
     8521 ccgactagca aaaagttgtt aagtaatgcc tggtttgact taaaacccaa cggttctagt
     8581 caattaaaga agatatttgg aaggaaggtt ttcgataacc ctaaatcaat tgatttggta
     8641 aaaagactaa tacaatttgc gactgaaaac aaagaagcga ttgtacttga ctcatttgca
     8701 gggagtggca ctactgctca tgcagtatta tcgcttaaca aacaagatgg agggaatcgt
     8761 cgtttcatcc ttattgagat ggaagaatat gcacatgaga ttaccgcaga acgcggatct
     8821 ttatcgattt gcatgcaagc taattcggtg gaaacgaggt catcatttcc ttccgaaaaa
     8881 acggttgcat ttaaatctta catatgtaat actttcaaag actacatttg taagatttga
     8941 tgtttgagtc ggctgaaaga tcgtacgtac caattattgt ttcgtgattg ttcaagccat
     9001 aacactgtag ggatagtgga aagagtgctt catctggtta cgatcaatca aatattcaaa
     9061 cggagggaga cgattttgat gaaaccagta acgttatacg atgtcgcaga gtatgccggt
     9121 gtctcttatc agaccgtttc ccgcgtggtg aaccaggcca gccacgtttc tgcgaaaacg
     9181 cgggaaaaag tggaagcggc gatggcggag ctgaattaca ttcccaaccg cgtggcacaa
     9241 caactggcgg gcaaacagtc gttgctgatt ggcgttgcca cctccagtct ggccctgcac
     9301 gcgccgtcgc aaattgtcgc ggcgattaaa tctcgcgccg atcaactggg tgccagcgtg
     9361 gtggtgtcga tggtagaacg aagcggcgtc gaagcctgta aaacggcggt gcacaatctt
     9421 ctcgcgcaac gcgtcagtgg gctgatcatt aactatccgc tggatgacca ggatgccatt
     9481 gctgtggaag ctgcctgcac taatgttccg gcgttatttc ttgatgtctc tgaccagaca
     9541 cccatcaaca gtattatttt ctcccatgaa gacggtacgc gactgggcgt ggagcatctg
     9601 gtcgcattgg gtcaccagca aatcgcgctg ttagcgggcc cattaagttc tgtctcggcg
     9661 cgtctgcgtc tggctggctg gcataaatat ctcactcgca atcaaattca gccgatagcg
     9721 gaacgggaag gcgactggag tgccatgtcc ggttttcaac aaaccatgca aatgctgaat
     9781 gagggcatcg ttcccactgc gatgctggtt gccaacgatc agatggcgct gggcgcaatg
     9841 cgcgccatta ccgagtccgg gctgcgcgtt ggtgcggata tctcggtagt gggatacgac
     9901 gataccgaag acagctcatg ttatatcccg ccgttaacca ccatcaaaca ggattttcgc
     9961 ctgctggggc aaaccagcgt ggaccgcttg ctgcaactct ctcagggcca ggcggtgaag
    10021 ggcaatcagc tgttgcccgt ctcactggtg aaaagaaaaa ccaccctggc gcccaatacg
    10081 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc
    10141 cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttaggatc ctgcctcgcg
    10201 cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct
    10261 tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc
    10321 gggtgtcggg gcgcagccat gacccagtca cgtagcgata gcggagtgta tactggctta
    10381 actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc
    10441 acagatgcgt aaggagaaaa taccgcatca ggcgctcttc cgcttcctcg ctcactgact
    10501 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
    10561 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
    10621 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
    10681 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
    10741 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
    10801 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
    10861 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
    10921 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
    10981 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
    11041 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga
    11101 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
    11161 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
    11221 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
    11281 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
    11341 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
    11401 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
    11461 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
    11521 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
    11581 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
    11641 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
    11701 ttaatagttt gcgcaacgtt gttgccattg ctgcaggcat cgtggtgtca cgctcgtcgt
    11761 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
    11821 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
    11881 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
    11941 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
    12001 tgcggcgacc gagttgctct tgcccggcgt caacacggga taataccgcg ccacatagca
    12061 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
    12121 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
    12181 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
    12241 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
    12301 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
    12361 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa
    12421 ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtcttc
    12481 aagaa
//
